ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed using antibody 031203. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.

ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed using antibody 031203 and control immunoglobulin G as indicated. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.

ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts using antibody 031203. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.